Native Human Collagen I protein is a Human Full Length protein, expressed in Native, with >95% purity and suitable for WB, SDS-PAGE.
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5 Publications for All applications
Acta biomaterialia 99:269-2832019
Donor age significantly influences the Raman spectroscopic biomolecular fingerprint of human pancreatic extracellular matrix proteins following collagenase-based digestion.
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Unspecified application
Reactive species
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Unspecified reactive species
Rebecca M Spiers et. al.
PubMed 31525537Neuron 99:702-719.e62018
Extracellular Matrix Components HAPLN1, Lumican, and Collagen I Cause Hyaluronic Acid-Dependent Folding of the Developing Human Neocortex.
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Unspecified application
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Unspecified reactive species
Katherine R Long et. al.
PubMed 30078576Cancer biology & therapy 19:904-9122018
A fragment of SPARC reflecting increased collagen affinity shows pathological relevance in lung cancer - implications of a new collagen chaperone function of SPARC.
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Unspecified application
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Unspecified reactive species
S N Kehlet et. al.
PubMed 30067436American journal of physiology. Heart and circulat 309:H1883-932015
Calpastatin overexpression impairs postinfarct scar healing in mice by compromising reparative immune cell recruitment and activation.
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Unspecified application
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Unspecified reactive species
Feng Wan et. al.
PubMed 26453333The British journal of dermatology 164:83-962010
Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy.
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Unspecified application
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Unspecified reactive species
F Syed,E Ahmadi,S A Iqbal,S Singh,D A McGrouther,A Bayat
PubMed 20849516
Key facts
- Purity
>95%
- Expression system
Native
- Tags
Tag free
- Applications
WB, SDS-PAGE
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/1000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Application SDS-PAGE | Reactivity Expected | Dilution info - | Notes - |
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2 products for Alternative Product
Target data
See full target information COL1A1
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Recommended products
Native Human Collagen I protein is a Human Full Length protein, expressed in Native, with >95% purity and suitable for WB, SDS-PAGE.
Key facts
- Purity
>95%
- Expression system
Native
- Tags
Tag free
- Applications
WB, SDS-PAGE
- Biologically active
No
- Accession
- P02452-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.003% Acetic acid
Sequence info
Amino acid sequence
- Accession
- P02452
- Protein length
Full Length
- Predicted molecular weight
139 kDa
- Nature
Native
Specifications
- Form
Liquid
- Additional notes
Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
General info
- Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
- Sequence similarities
Belongs to the fibrillar collagen family.
- Post-translational modifications
Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
- Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
- Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
- Associated diseases and disorders
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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1 product image
Western blot - Native Human Collagen I protein (ab7533)
DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.
Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
Other Band(s): Collagen Type I splice variants and isoforms.
All lanes: Western blot - Anti-Collagen I + Collagen III antibody (Anti-Collagen I + Collagen III antibody ab34710) at 1/1000 dilution
All lanes: Western blot - Native Human Collagen I protein (ab7533) at 0.05 µg
Secondary
All lanes: DyLight™ 649 anti-rabbit secondary antibody at 1/20000 dilution
Predicted band size: 139 kDa
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Product protocols
For this product, it's our understanding that no specific protocols are required. You can:
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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